01 February 2005
Evidence of the glyoxylate cycle in the liver of newborn rats
Igor G. Morgunov, Maria N. Kondrashova, Svetlana V. Kamzolova, Alexander P. Sokolov, Nadezhda I. Fedotcheva, Tatiana V. FinogenovaMed Sci Monit 2005; 11(2): BR57-60 :: ID: 14143
Abstract
Background:It is generally accepted that the glyoxylate cycle exists in microorganisms and higher plants but absent in higher animals. The hypothesis of the glyoxylate cycle in the tissues of higher animals with a high level of physiological activity was first proposed by Kondrashova and Rodionova in 1971. The goal of this work was to verify this in newborn rats, which possess a 2.5-fold higher physiological activity and oxygen consumption rate than adult rats.Material/Methods:Newborn (7-day-old) and adult rats were used for this experiment. The activities of the key enzymes of the glyoxylate cycle (isocitrate lyase and malate synthase) were measured by HPLC and spectroscopic methods.Results:The activities of isocitrate lyase and malate synthase were found in the liver homogenates prepared from newborn rats, but not from adult rats. The activities of the enzymes common to both the Krebs cycle and the glyoxylate cycle (citrate synthase, aconitase, and malate dehydrogenase) were 20–40% higher in newborn than in adult rats.Conclusions:These data indicate the existence of the glyoxylate cycle in animal tissues.
Keywords: Aconitate Hydratase - metabolism, Citrate (si)-Synthase - metabolism, Glyoxylates - metabolism, Isocitrate Lyase - metabolism, Malate Dehydrogenase - metabolism, Malate Synthase - metabolism, Aconitate Hydratase - metabolism, Aging - physiology, Animals, Newborn, Chromatography, High Pressure Liquid, Citrate (si)-Synthase - metabolism, Citric Acid Cycle, Glyoxylates - metabolism, Isocitrate Lyase - metabolism, Liver - metabolism, Malate Dehydrogenase - metabolism, Malate Synthase - metabolism, Rats, Wistar
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
15 Apr 2024 : Laboratory Research
The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in OsteoarthritisMed Sci Monit In Press; DOI: 10.12659/MSM.943738
07 Mar 2024 : Clinical Research
Knowledge of and Attitudes Toward Clinical Trials: A Questionnaire-Based Study of 179 Male Third- and Fourt...Med Sci Monit In Press; DOI: 10.12659/MSM.943468
08 Mar 2024 : Animal Research
Modification of Experimental Model of Necrotizing Enterocolitis (NEC) in Rat Pups by Single Exposure to Hyp...Med Sci Monit In Press; DOI: 10.12659/MSM.943443
18 Apr 2024 : Clinical Research
Comparative Analysis of Open and Closed Sphincterotomy for the Treatment of Chronic Anal Fissure: Safety an...Med Sci Monit In Press; DOI: 10.12659/MSM.944127
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952