22 February 2002
mRNA expression of EGF receptor ligands in atrophic gastritis before and after Helicobacter pylori eradication
Wolfram Domschke, Kazimierz Rembiasz, Ronald Assert, Jan Konturek, Uwe Schiemann, Stanisław Konturek, Aadreas PfeifferMed Sci Monit 2002; 8(2): CR53-58 :: ID: 420941
Abstract
BACKGROUND: Epidermal growth factor (EGF) receptor ligands (EGFRL) includingtransforming growth factor alpha (TGF-alpha), amphiregulin, and heparin binding-EGF (HB-EGF) are involvedin gastric mucosal repair in chronic gastritis. Their mRNA expression has been shown to be upregulatedafter Helicobacter pylori (H.p.)-eradication but little is known about this gene expression in atrophicgastritis. The purpose of our study was to investigate EGFRL mRNA expression in gastric mucosa of patientswith atrophic gastritis before and after H.p.-eradication. MATERIAL/METHODS: Antral mucosal biopsieswere obtained during endoscopy in 10 H.p. positive patients with atrophic gastritis and in 10 H.p. negativecontrols with intact mucosa. Total RNA of antral biopsies was extracted and RT-PCR was performed, thePCR-products being measured densitometrically. Values were compared with mRNA expressions in H.p. negativeantral mucosa (n=10). RESULTS: Gastric biopsies revealed mRNA expression for TGF-alpha, amphiregulinand HB-EGF, both in H.p. positive atrophic antritis and in H.p. negative healthy mucosa. The mRNA expressionof TGF-alpha in atrophic gastritis was significantly upregulated after H.p.-eradication, whereas thatof amphiregulin did not change after this eradication. Expression of HB-EGF mRNA was higher in H.p.-infectionthan after H.p.-eradication or in H.p. negative healthy subjects. CONCLUSIONS: H.p. positive atrophicgastritis is associated with differential mRNA expression of EGF receptor ligands. H.p.-eradication inthis entity leads to unequal changes of these growth factor expressions compared to chronic active gastritiswithout atrophy.
Keywords: Base Sequence, DNA Primers, Epidermal Growth Factor, Gastritis, Atrophic, Glycoproteins, Growth Substances, Helicobacter Infections, Helicobacter pylori, Intercellular Signaling Peptides and Proteins, Ligands, RNA, Messenger, Receptor, Epidermal Growth Factor, Reverse Transcriptase Polymerase Chain Reaction, Transforming Growth Factor alpha
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
06 Mar 2024 : Clinical Research
Comparison of Outcomes between Single-Level and Double-Level Corpectomy in Thoracolumbar Reconstruction: A ...Med Sci Monit In Press; DOI: 10.12659/MSM.943797
21 Mar 2024 : Meta-Analysis
Economic Evaluation of COVID-19 Screening Tests and Surveillance Strategies in Low-Income, Middle-Income, a...Med Sci Monit In Press; DOI: 10.12659/MSM.943863
10 Apr 2024 : Clinical Research
Predicting Acute Cardiovascular Complications in COVID-19: Insights from a Specialized Cardiac Referral Dep...Med Sci Monit In Press; DOI: 10.12659/MSM.942612
06 Mar 2024 : Clinical Research
Enhanced Surgical Outcomes of Popliteal Cyst Excision: A Retrospective Study Comparing Arthroscopic Debride...Med Sci Monit In Press; DOI: 10.12659/MSM.941102
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952