Errate: Promoting Role of Long Non-Coding RNA Small Nucleolar RNA Host Gene 15 (SNHG15) in Neuronal Injury Following Ischemic Stroke via the MicroRNA-18a/CXC Chemokine Ligand 13 (CXCL13)/ERK/MEK Axis

Tiezhu Guo; Yueting Liu; Xinliang Ren; Wei Wang; Hanrui Liu

doi:10.12659/MSM.938473

28 September 2022: Erratum

Errate: Promoting Role of Long Non-Coding RNA Small Nucleolar RNA Host Gene 15 (SNHG15) in Neuronal Injury Following Ischemic Stroke via the MicroRNA-18a/CXC Chemokine Ligand 13 (CXCL13)/ERK/MEK Axis

Tiezhu Guo¹, Yueting Liu², Xinliang Ren¹, Wei Wang¹, Hanrui Liu^3*

DOI: 10.12659/MSM.938473

Med Sci Monit 2022; 28:e938473

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Abstract

ABSTRACT: The figure 3D was published as figure 3E. The correct figure 3E is provided below. Reference: Tiezhu Guo, Yueting Liu, Xinliang Ren, Wei Wang, Hanrui Liu. Promoting Role of Long Non-Coding RNA Small Nucleolar RNA Host Gene 15 (SNHG15) in Neuronal Injury Following Ischemic Stroke via the MicroRNA-18a/CXC Chemokine Ligand 13 (CXCL13)/ERK/MEK Axis. Med Sci Monit 2020; 26:e923610; DOI: 10.12659/MSM.923610

Keywords: Cell Line, Tumor, Cell Proliferation, Chemokine CXCL13, Chemokines, CXC, Gene Expression Regulation, Neoplastic, Humans, ischemic stroke, Ligands, MicroRNAs, Mitogen-Activated Protein Kinase Kinases, RNA, Long Noncoding, RNA, Small Nucleolar

Figures

SNHG15 serves as a ceRNA for miR-18a to mediate THBS2 expression. (A, B) Sub-cellular localization of SNHG15 in N2a cells was determined by a RT-qPCR (A) and a FISH assay (B). (C, D) Binding relationships between miR-18a and SNHG15 (C), and between miR-18a and the 3′-UTR of CXCL13 mRNA (D) were predicted on Starbase (http://starbase.sysu.edu.cn/) and validated through dual-luciferase reporter gene assay. (E) Contents of total ERK/MEK and phosphorylated ERK/MEK in N2a cells was quantified by ELISA kits. F, phosphorylation of ERK1/2 and MEK1/2 was determined by Western blot analysis. Data were shown as mean±SD based on 3 independent experiments. * p<0.05. In panels A and F, data were analyzed using two-way ANOVA, while data in panels C–E were analyzed using one-way ANOVA, and Tukey’s multiple comparison test was used for post hoc analysis.

Figure 3. SNHG15 serves as a ceRNA for miR-18a to mediate THBS2 expression. (A, B) Sub-cellular localization of SNHG15 in N2a cells was determined by a RT-qPCR (A) and a FISH assay (B). (C, D) Binding relationships between miR-18a and SNHG15 (C), and between miR-18a and the 3′-UTR of CXCL13 mRNA (D) were predicted on Starbase (http://starbase.sysu.edu.cn/) and validated through dual-luciferase reporter gene assay. (E) Contents of total ERK/MEK and phosphorylated ERK/MEK in N2a cells was quantified by ELISA kits. F, phosphorylation of ERK1/2 and MEK1/2 was determined by Western blot analysis. Data were shown as mean±SD based on 3 independent experiments. * p<0.05. In panels A and F, data were analyzed using two-way ANOVA, while data in panels C–E were analyzed using one-way ANOVA, and Tukey’s multiple comparison test was used for post hoc analysis.

Reference

. Guo Tiezhu, Liu Yueting, Ren Xinliang, Wang Wei, Liu Hanrui, RPromoting Role of Long Non-Coding RNA Small Nucleolar RNA Host Gene 15 (SNHG15) in Neuronal Injury Following Ischemic Stroke via the MicroRNA-18a/CXC Chemokine Ligand 13 (CXCL13)/ERK/MEK Axis: Med Sci Monit, 2020; 26; e923610, doi: 10.12659/MSM.923610

Link to the original article: https://medscimonit.com/abstract/index/idArt/923610

Figures

Figure 3. SNHG15 serves as a ceRNA for miR-18a to mediate THBS2 expression. (A, B) Sub-cellular localization of SNHG15 in N2a cells was determined by a RT-qPCR (A) and a FISH assay (B). (C, D) Binding relationships between miR-18a and SNHG15 (C), and between miR-18a and the 3′-UTR of CXCL13 mRNA (D) were predicted on Starbase (http://starbase.sysu.edu.cn/) and validated through dual-luciferase reporter gene assay. (E) Contents of total ERK/MEK and phosphorylated ERK/MEK in N2a cells was quantified by ELISA kits. F, phosphorylation of ERK1/2 and MEK1/2 was determined by Western blot analysis. Data were shown as mean±SD based on 3 independent experiments. * p<0.05. In panels A and F, data were analyzed using two-way ANOVA, while data in panels C–E were analyzed using one-way ANOVA, and Tukey’s multiple comparison test was used for post hoc analysis.

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